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KMID : 0377619710210020129
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Korean Jungang Medical Journal
1971 Volume.21 No. 2 p.129 ~ p.139
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Effect of Heating of Bacterial Extract at Various pH Values on Phage Hemagglutination
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Abstract
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The author investigated the phage hemagglutination-forming ability of bacterial substance, extracted in normal saline by heating at 100°C for one hour, heated at various pH values. For this experiment, the Klebsiella phage host cell system and red cell of goat were used and obtained the following results.
When the bacterial substance is heated at 100°C for 10 minutes at pH 6.0 and 7.0, the phage hem agglutination-forming ability was not changed, while the phage hem agglutination was not formed if it is heated at pH 2.04.0. The phage hem agglutination became gradually weaker when the pH of bacterial substance for heating increase to alkaline side.
On heating the bacterial substance at acid pH, the phage hem agglutination forming ability became gradually weaker according to increasing of temperature and, when it is heated at 100°C for 10 minutes, the phage hem agglutination was completely or almost not formed.
At weak alkaline pH, the phage hem agglutination forming ability of bacterial substance became a little weaker if the heating temperature increase, while, at strong alkaline pH, the heating quantities did not show any special influence and the bacterial substance still formed, in, some degree, phage hem agglutination
even though heated for 10 minutes in the autoclave of 15 pounds pressure.
The sodium chloride did not protect the phage hem agglutination forming ability of bacterial substance under heating.
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